WebThe determination of creatinine has been developed using Jaffé reaction between the creatinine and picric acid in alkaline condition. The alkaline picrate color on μ PAD changed from yellow to orange in the presence of creatinine and the orange intensity is directly proportional to the creatinine amount in a linearity range of 0.20-6.0 mM as a detection … WebPlasma creatinine may be measured using either chemical or enzymatic methods. Most chemical methods utilize the Jaffe reaction. In this method creatinine reacts with picrate ion in an alkaline medium to produce an orange–red complex. The Jaffe reaction is not entirely specific for creatinine. Substances such as ascorbic acid, high glucose ...

Jaffé. Colorimetric - kinetic - SPINREACT

WebIn the Jaffe reaction, a red-orange chromogen is formed when creatinine reacts with Picric acid Substances known to increase results when measuring creatinine by the Jaffe reaction include all of the following except ascorbic acid bilirubin glucose alpha- keto acids Webby kinetic Jaffe’s and enzymatic method.The Jaffe’s method for serum creatinine determination is based on the principle thatpicric acid in an alkaline medium reacts with creatinine to form an rahmgulasch mit champignons

[A kinetic method of determining creatinine using the Jaffé reaction]

WebPlasma creatinine may be measured using either chemical or enzymatic methods. Most chemical methods utilize the Jaffe reaction. In this method creatinine reacts with picrate ion in an alkaline medium to produce an orange–red complex. The Jaffe reaction is not … WebThe normal serum creatinine (sCr) varies with the subject's body muscle mass and with the technique used to measure it. For the adult male, the normal range is 0.6 to 1.2 mg/dl, or 53 to 106 μmol/L by the kinetic or enzymatic method, and 0.8 to 1.5 mg/dl, or 70 to 133 μmol/L by the older manual Jaffé reaction. WebDec 22, 2016 · Both are colorimetric methods. In Jaffe methods, serum creatinine reacts with picrate to give a yellow-orange colour that can be quantified. This reaction is, however, not fully specific, as picrate can also react with other components, known as pseudo-chromogens (acetoacetate, pyruvate, keto-acids, proteins, glucose, and ascorbic acid) … rahmings medical