Phis2.1 vector

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August undefined, 2024

Webb22 juli 2013 · Description. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically … http://www.biofeng.com/zaiti/jiaomu/pYES2.html

Construction of yeast one-hybrid library for screening suspensor ...

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Addgene - Analyze Sequence

http://www.yrgene.com/documents/vector/matchmaker_library_constructionscreening_kits_user_manual.pdf http://www.honorgene.com/uploads/files/202411/6181dfe1813d2.pdf Webb19 sep. 2024 · The pHIS2 vector carries the TRP1 nutritional marker for selection in yeast. The transformed yeast cells were cultured on SD/-Trp-Leu medium and SD/-Trp-His-Leu medium supplemented with 10 mM or 30 mM 3-amino … can lithotripsy harm kidneys

Transcription Factor-Centered Yeast One-Hybrid Assay

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WebbUnique restriction sites are in bold. pHIS2.1 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the Matchmaker™ One-Hybrid Library Construction & Screening Kit (Cat. No. 630304). Webb1 juni 2024 · Clone the products of the PCR reactions carried out in step 3 into the pHIS2 T-vector obtained in step 5. The T-A ligation system includes 0.2 μg of T-vector of pHIS2, … fix blinking cursor windows 10WebbVectors in Use. N or C-terminal His tag. Parallel vectors 1 & 3 contain N-terminal His (pET22), MBP (pMal) and GST (pGex) with similar MCS for easy cloning. Parallel vector 2 contains N and C-terminal His (pET22), … can litigants in person claim costs

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Phis2.1 vector

3-AT(3-氨基-1,2,4-三唑)（ZCS104）-北京庄盟国际生物基因科技有 …

Webb12 apr. 2024 · 目的：筛选与启动子pHIS2-A互作的蛋白。. 方法：以启动子A构建到酵母单杂交启动子载体pHIS2，文库质粒为pGADT7-中山杉文库。. 材料：. 1．启动子克 … WebbStandard cloning vector (phagemid excised from lambda ZAPII). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript II KS(+) ... The 1-base overhangs …

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Webb20 feb. 2024 · The full-length cDNA of TcbZIP60 was cloned into the pGADT7 vector used for homologous recombination, and the promoter sequences of TcCHS and TcAOC were cloned separately into the pHis2.1 vector. The resultant pGADT7: TcbZIP60 plasmid was co-transformed into yeast strain Y187, together with pHis2.1: TcCHS or pHis2.1: TcAOC , … Webb1 mars 2024 · The full length CDS of VvWRKY30 was inserted into pGADT7 vector. The W-BOX, isolated from VvGolS1 (KX545686) promoter, was constructed into pHIS2.1 …

WebbBackground Rice transcription regulator OsWRKY13 influences the functioning of more than 500 genes in multiple signalling pathways, with roles in disease resistance, redox homeostasis, abiotic stress responses, and development. Results To determine Webb11 juli 2014 · 常用载体及通用测序引物,测序通用引物,华大测序通用引物,载体通用引物,petduet 1测序引物,通用引物,测序引物,pet28a测序引物,m13通用引物,细菌通用引物,病毒通用引物. VectorPrimer (F)Primer (R)抗性载体大小 (bp)EZ-TM13-47RV-MAmp+2984LZRS-RfALZRS-FLZRS-R11100p3xFLAGMV14CMV30 (834bp)CMV24 ...

WebbBackground Drought is ampere major abiotic stress factors that reduces agricultural productivity. GRAS transcription factors exist plant-specific proteins that play diverse roles in plant development. However, that functions of a count of GRAS genes identified in oats are unknown, specifically the GRASS genes related for fruit drought resistance have not … WebbChina Agricultural University (CAU, Chinese: 中国农业大学; pinyin: Zhōngguó Nóngyè Dàxué; abbreviated 农大/Nóngdà) is a university in Beijing, People's Republic of China …

Webb一、载体概念. 1. 载体，DNA的搬运工. 载体：在基因工程重组DNA技术中，将DNA片段（目的基因）转移至受体细胞的一种能自我复制的DNA分子。. 2. 载体，基因密码承载者. 载 …

WebbUSD $185.00. Matchmaker Insert Check PCR Mix 1 is a 2X pre-mix that allows quick and easy PCR directly from yeast colonies. The Mix contains a PCR polymerase, primers, dNTPs and buffer; simply add cells to the mix … fix blinking monitorWebbpHIS2 vector (V001297#) Basic Vector Information. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The … can lithromantic people dateWebb8 juli 2024 · pHis2 编号载体名称北京华越洋生物VECT2110 pHis2 pHis2 载体基本信息出品公司: Clontech 载体名称: pHis2 质粒类型: 酵母单杂交载体高拷贝/低拷贝: -- 启动子: -- … fix blinking mousehttp://www.biofeng.com.cn/zaiti/jiaomu/pHis2.html fix blisterWebbLearn Biology Online. Biology Online is the world’s most comprehensive database of Biology terms and topics. Since 2001 it has been the resource of choice for professors, students, and professionals needing answers to Biology questions. Search over 75,000+ terms, news, insights, discoveries, and trends in Biology below: can litleo be shiny pokemon goWebbA yeast one-hybrid library was constructed to screen the binding proteins of suspensor specific cis-element. In this study, five repeats of suspensor specific cis-element … can litten be shinyhttp://wolfson.huji.ac.il/expression/bac-vectors.html can litter boxes host fleas