Phosphopantetheine binding

Author: hveo

August undefined, 2024

Webphosphopantetheine prosthetic group in the T domain to yield tyrosyl-S-NovH (Figure 2). It has been our premise that all subsequent modification steps leading to aminocoumarin … WebApr 16, 2014 · Protein has 1 cofactor binding site: pantetheine 4'-phosphate ( UniProtKB Rhea CHEBI:47942 ) By similarity Note: Binds 2 phosphopantetheines covalently. By similarity Pathway Siderophore biosynthesis; mycobactin biosynthesis. GO annotations Expand table Complete GO annotation on QuickGO Keywords Molecular function # Ligase

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WebUsing Escherichia coli LipB as a model enzyme, we show here that the octanoyl-transferase mainly recognizes the 4'-phosphopantetheine-tethered acyl-chain of its donor substrate and weakly binds the apo-acyl carrier protein. We demonstrate LipB can accept octanoate from its own ACP and noncognate ACPs, as well as C8-CoA. WebApr 15, 1999 · The hydrogen bond between the amide of Met74 and the carboxylic oxygen at the elbow of the pantetheine arm stabilizes the bent configuration. In the free protomer, residues 72–74 hinder binding of 4′-phosphopantetheine and would result in steric conflict between Met74 and carboxylic oxygen, thus entailing backbone movement prior to binding. chinese labor rates and trends

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WebPhosphopantetheine-binding protein (Streptomyces rimosus subsp. rimosus ATCC 10970) Cite Download Contents 1 Sequence 2 Interactions and Pathways 3 Biochemical … WebSep 29, 2024 · A 4'-phosphopantetheine prosthetic group is attached through a serine. This prosthetic group acts as a a 'swinging arm' for the attachment of activated fatty acid and … WebPresumably, the phosphopantetheinyl group of coenzyme A is transferred to FenB by the same mechanism and subsequently results in the binding of the [ 14 C]isoleucine to the enzyme. We found that approximately 13% of FenB expressed in E. coli bound to the amino acid. Biochemical characterization of recombinant FenB. grand palms assisted living

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WebPFAM: phosphopantetheine-binding; KEGG: mxa:MXAN_2796 non-ribosomal peptide synthetase (90 aa) Predicted Functional Partners: PC1_0576. D-alanine--poly … WebPhosphopantetheine tethering is a posttranslational modification that takes place on the active site serine of carrier proteins 138 – ‘acyl carrier proteins (ACPs) and peptidyl … chinese laborers in usWebMay 30, 2024 · To characterize the Mab PPAT ligand binding propensities, we determined crystal structures of Mab PPAT in complex with natural substrates ATP and 4′-phosphopantetheine (PhP). ATP occupies a positively charged pocket in the Mab PPAT substrate binding groove with the adenine ring making hydrogen-bond contacts with side … chinese kylin

"WebJul 17, 2024 · A phosphopantetheine-binding-induced activation mechanism To verify our hypothesis, we determined the crystal structure of the CAR sr PCP–reductase (PCP–R) … " - Phosphopantetheine binding

Phosphopantetheine binding

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WebAn object of the present invention is to provide a microorganism that efficiently produces EPA and a method for producing EPA using the microorganism. The present invention relates to a microorganism having an ability to produce docosahexaenoic acid (DHA), wherein the microorganism contains a protein composed of an amino acid sequence in … Web2.8 2.9 Structures of essential biomolecules pantothenate, phosphopantetheine, coenzyme A, and acyl-ACP Biosynthetic pathway of Coenzyme A Sequence similarity of CoA biosynthetic enzymes among several species Selected CoA biosynthetic pathway inhibitors The membranes of gram-negative bacteria Biosynthetic pathway of lipid A

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WebOct 5, 2007 · Together with docking simulations that identified a potential phosphopantetheine binding groove, the structural and functional studies reveal that the C9 specificity is a result of active site geometry and substrate ring constraints. The results lay the foundation for the design of novel aromatic polyketide natural products with different ... WebBinding to phosphopantetheine, the vitamin pantetheine 4'-(dihydrogen phosphate). Source: GOC:vw, GOC:mah Comment None History See term history for GO:0031177 at QuickGO …

Webcytoskeleton and flagella extracellular region protein binding, modification* transmembrane transport cellulose binding calcium ion binding microtubule-based movement calcium channel complex NAD(P)H oxidase activity small GTPase signal cell regulation and signalling* proteolysis* positive regulation of GTPase activity membrane kinesin complex

WebFeb 1, 2024 · The reaction mechanism of PPAT involves a nucleophilic attack by 4′-phosphate of phosphopantetheine on the α-phosphate of ATP in an in-line displacement mechanism, where both the substrates are positioned in a single line. This results in a short lived intermediate pentacovalent transition state. WebJan 4, 2024 · Crystal structures of the PCP-reductase didomain reveal that phosphopantetheine binding alters the orientation of a key Asp, resulting in a productive orientation of the bound nicotinamide. This ensures that further reduction of the aldehyde product does not occur. Combining crystallography with small-angle X-ray scattering …

WebFeb 9, 2024 · Binding poses of the natural substrates 4′-phosphopantetheine (PPSH) and ATP are shown for comparison. C) Hydrogen-bonding interactions and hydrophobic …

http://amigo.geneontology.org/amigo/term/GO:0031177 grand palms event center chaska mnWebSep 4, 2024 · phosphopantetheine binding pocket, away from the solvent channel, forming a hydrogen bond with . Val73, and interacting via water molecules (not shown) with Gly71, Val74 and Asn105 (Figure 2C). chinese labor built the railroadWebJun 6, 2024 · Unique CFP (cysteine-free protein; 120 aa) has been identified as an extraordinary virulence factor in Beauveria bassiana (Cordycipitaceae), a main source of wide-spectrum fungal insecticides. Its homologs exclusively exist in wide-spectrum insect pathogens of Hypocreales, suggesting their importance for a fungal insect-pathogenic … chinese lacquered sewing tablehttp://nextgen.mgh.harvard.edu/IlluminaChemistry.html chinese labor strikeWebJan 3, 2024 · Lipoic acid is a sulfur containing cofactor, indispensable for the function of several metabolic enzymes. In microorganisms, lipoic acid can be salvaged from the surroundings by Lipoate protein ligase A (LplA), an ATP-dependent enzyme. Alternatively, it can be synthesized by the sequential action of Lipoate protein ligase B (LipB) and Lipoyl … chinese labour law yan wangWebNDA 021160/S-015 Page 2 with PhosLo. Progressive hypercalcemia due to overdose of PhosLo may be severe as to require emergency measures. Chronic hypercalcemia may … grand palms community pembroke pinesWebPhosphopantetheine-binding protein (Pectobacterium carotovorum PC1) - STRING interaction network Nodes: Network nodes represent proteins splice isoforms or post-translational modifications are collapsed, i.e. each node represents all the proteins produced by a single, protein-coding gene locus. Node Color colored nodes: grand palm resort myrtle beach south carolina